Did you try performing a different OOB, like shown
here?
1:55 Did you try running ahead without hiding from the arrows?
Some moments, like 3:56, 4:23, some inventory selections, 5:24, cutscene slow skip and other ones look slow to me.
I don't know why you always do a step back before turning around.
Killing the Gatling Man looks less optimized than killing Krauzer. It's like by that point you finally got familiar with the control.
Some turns and camera angles look a little slow too. I got the feeling like you didn't have enough hands or keyboard input to do them better. Did you use TAS Input? Also, check "Aiming" paragraph in this
submission, it might be useful to optimize turns.
Reloading the sniper rifle can be improved if you take damage during the reloading animation. I don't remember if it works with CGI cutscenes, but you should try reloading it before fighting Krauzer to see if the cutscene fast-forwarded the animation.
What memory card you use for the submission? Did you download it from somewhere or did you use the one that's verified from that
submission ("Playing the .dtm file on Dolphin" paragraph)?
In short, you first submission is good, but I found it not optimised. Please do an updated version. I don't think it will take long to redo it.