I'm glad to see this game on the workbench, but I have some wellfounded concerns.
I posted on the Boogerman thread
a while back a short WIP of the first 3 levels with some information that I think you could have used here.
First of all, jumping on trampolines slows you down, and you seem to jump on quite a lot of them for no real reason.
Secondly, gathering too many plungers increases your time in the bonus screen. For example at the end of stage 1-2, I was 76 frames ahead. But because I only collected one plunger for that level, and you collected 8, I was able to start 1-3 119 frames ahead.
Finally I stated that the fire powerup causes more damage to enemies/bosses that you don't seem to have used. I think you could cut down considerably on the time by using this powerup.
Another thing to note; you might want to add different things to make the movie entertaining. For example, I found that you can slide on branches with a stuck animation, while not losing any speed.
I'm only saying this so that maybe you will improve the run, a game in which I really want to see done.
As for right now I won't vote, but I have no intention of doing the run myself because I have concerns about the ROM. Various glitches occurred when I tried to do this myself. But I do wish you good luck on improving this game!