This is a 2 frame improvement over the previous movie. The improvement comes from an earlier first hit on the Dark Link fight. No other inputs were changed.
If you need a rundown of the route for this game, I would suggest reading the previous submissions, as this one made no changes in its route.
: Given that the same improvement works in FCEUX, it's safe to say this improvement isn't related to emulation differences at all.
Despite this improvement being small, there doesn't look to be any obvious places to improve from here.
Given the previous run has high ratings and this one has really positive reception as well (being 90% the same run), not to mention all the weird... fast... glitchy... stuff... that happens in the run, it's a no brainer what to do with it.