P2 boss 7 frames faster with jumping start (first frame)
p4l1 dropping from first platform is 2 frames faster than instead of dropping from first frame and dropping again, but no time save (whatever frame rule)
1 frame faster P1L1 (sub x optimal) but doesn't matter, probably there is a frame rule (which would also explain the manual "insert 1 frame and remove 1 frame before and after a section" this isn't the reason as that one depends on lag frames)
16 frames faster P2 boss by climbing the wall on the left and jumping off to get to the green goo earlier
edit: this movie includes the P3 boss 2 lag frames improvement
Nearly 100% optimized but didn't checked subpixels
Note
there can be random 2 frames of saving or loss between levels (and cinematics)
edit2: you can also insert 1 frame before sections and delete 1 frame after. not sure if this has any potential use or why is it works in the first place...
planet 1 level 1 - only final dmg taken matters
planet 3 level 1 - not sure if it's fastest on last enemy
all lag frames eliminated except for...
planet 3 level 3 - jumping asap triggers enemy earlier, so this is faster than jumping later
planet 3 boss - 9 lag frames is best for me
planet 5 level 1 - mixing vertical and horizontal optimization but 1 lag frame is still there
So need to check subposes, p3l3, p3boss, p5l1.
edit: p3boss can be 2 frames faster with lag optimization (removing R at specific frames)
Implements all known tricks except not useful ones (front doors/object interaction and walking away) and doesn't optimizes lag frame saves (random lag frames depending on when you move the cursor)