In accordance with standard procedure, Samus discarded all of the items she collected on previous adventures before embarking on this one. This run aims for 100% item collection in the fastest time.

Super Metroid has a wide variety of time-saving glitches to exploit, many of which are explained on the Super Metroid Tricks page.

The in-game time of this run is 39 minutes 55 seconds, but since game timers have never really been good at rounding things up, it is shown as 00:39 after the game has been completed.