I've got a little time today to work on things, and I've started back at the Stage 1 2nd boss, where I first saw a problem. I managed to beat him before he went into his 2nd hop, which allows a big chunk of time to be saved.
Through the 1st stage, just that one minor change saved 167 frames. I might lose a few of those in Stage 2, as I think, based upon what I've played around with Stage 4, that I might need that boxed heart, which increases my HP level by 1, in order to save some time. Not sure at this point.
Edit: Through stage 2, even with the detour for the heart, I'm now 207 frames ahead. I picked up a few frames by improving the Stage 2 boss fight by doing some stylized damage, and cutting out the excess I did as he ran to the left.
Back to Stage 3 I go.
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Stage 3 is redone. 333 frames ahead of WIP3. ^^ Not sure where I gained all those frames, since most of it is autoscroller. >_>;;
WIP4