This is my improvment.
http://tasvideos.org/userfiles/info/25500042330572150
This run is 128 frames faster. Don't add me to the co-author, and I don't plan to submit it, too. Try your best and beat me, then I will vote yes.
Round1.
11 frames saved. Because this game has the 11 frames rule. 11 frames rule address: 0x0020.
Don't fire the 2nd smoke too close to the 1st at the begining, because when these two shots hit the DK together, DK only be hit once. Watch the DK Y axis address: 0x0368 & 0x0371. Gay's and MESHUGGAH's has the same mistake.
If you want to improve, find 11 frames imrpovment. I have found 6 frames, but nothing changed.
Round2.
110 frames saved total. Manipulate luck the bee, find a better death way. If you try to improve this round, you should find more than 8 frames.
Round3.
112 frames saved total. Not the 11 frames rule? When beat DK, I'm 127 frames faster. But when countdown the time, this is 10-frame-rule, so it changed to 120. But when countdowm the flower, it's 16 frames rule, oh dammit! Only 112 frames left! I guess if it can be found 3 more frames saved when beating DK, it could be saved 16 frames.
Round4.
128 total. Gay is slower than MESHUGGAH. I improved MESHUGGAH's 3 frames, the better jump.