Alright, i think i've overdone myself now. 22:40 run, level 5 route (its autoscrolling, levle 4 isnt, so, even on a timeattack level 4 being 30 seconds faster, i dont think that its a good choice for a speedrun)
I lost 3 lives total during levels, here is how much time i lost by having to backtrack due to lost lives:
level 3 =~ 15 seconds (1 life lost)
level 9 =~ 16 seconds (1 life lost)
level 12 =~ 45 seconds (1 life lost)
Also lost 1 life at boss of level 11, one life at dark queen, and 1 life after dark queen fight (after stopped recording). But its hard to measure how much time was lost there, so i'm not counting it.
So, the way i played the time would have been 21:24~ if i hadnt lost any lives. The most improveable place is level 12. I dont think i can make the other levels much better (besides some missed punches).
Here is the video:
http://www.angelfire.com/ex/uobt/battletoads-u-speedrun-foda-v4.zip