OK, so a list of mistakes I've seen in the speed-TASed portion of the run (I also noticed a few in the gametime portion, but those will probably be redone anyway for the gametime TAS; this list may be incomplete, I got kind-of numb to all the mistakes after a while):
* (robe?)
* (too many potions?)
* Entering invalid/incorrect commands just before the fight with Narodnaja. Entering ' ' just wastes realtime, whereas entering 'B' destroys the legitimacy of the run (it's a nethack-tas-tools cheat code for manipulating level entry positions, and something that's meant to be loadstated away each time it's used).
* Killing the small mimics wasted a lot of time for no gain I'm aware of (was that for alignment-fixing? if so, killing them makes sense, but they could have been killed rather faster, and you wasted an action afterwards to trigger teleportitis, rather than wasting it in a low-lag area or via polyform).
* Walking from dlvl4 to dlvl6 manually (and onwards via teleport to the stairs rather than levport) surely has to waste time. If you're looking for enemies to kill, teleport to an area with a lot (a zoo, or the like) and kill them quickly and stealthily there.
* Autopicking up items you don't need (like the elf mummy's wrapping on dlvl 7) wastes realtime due to the need to print text describing what you picked up.
* Given that you're moving on the "descension run" down from level 4, you should be in a slower-than-normal polyform to reduce the number of screen redraws needed compared to the number of turns before 2000 burned, unless you're having problems fitting everything in (which you shouldn't be). This looks like you're being excessively cautious and haven't planned out the rest of the run.
* On dlvl 8, you're clearly using walking rather than waiting to trigger a teleport. Although I'm not 100% certain, the extra vision calculations and screen redraws imply to me that this is a more laggy option than waiting would be, and will come to the same thing. (You could also use travel + a low runmode to reduce the number of screen redraws, if you need to move for some reason; I'd be more willing to believe that this is faster than waiting, than that your current route is.)
* The Wizard of Yendor fight on dlvl 11 takes much longer than it should in a TAS. You have a ton of alternatives that could oneshot the Wizard; some might be slower due to needing to enter extra text, but I find it hard to believe that all are slower than the brute force method. (For example, perhaps you should have been coming down here in cockatrice rather than jabberwock form.)
* Which reminds me: why are you using jabberwock form for a descension run, given that it prints an extra message at every stairway?
* Minotaur form on dlvl 14 is faster than the neutral speed of 12. This forces you to burn more turns later.
* During the throne room fight, most of your actions print multiple lines of messages. When aiming for realtime, you should skip all but the first line with Esc. This is an obvious timesaver and it looks very suspicious that you aren't doing it. (I didn't skip most messages in the stretch of my input you used, but that's because I was aiming for gametime and messages don't count against that.)
* An even worse case of the same thing is not pressing Esc when you get paralyzed. Getting paralyzed is reasonable from the point of view of skipping turns, but if you're not skipping messages too and thus allowing the game to run at full speed, you don't get a benefit, it just purely hurts.
* You went out of your way to pick up items earlier (such as the extra leather armors), then you went out of your way to drop them on dlvl 14, and then you didn't use them. This is clearly inefficient and a waste of your time.
* Killing everything in the throne room was unnecessary, there's easily enough monsters there to cap you on alignment. You should have just killed the ones that had teleportation scrolls. (Yes, I know nethack-tas-tools doesn't have a memory watch feature for that yet, but an optimized TAS would have added one rather than performing a lot of unnecessary kills, or at least experimented via savestates.)
* I'm assuming you sat on the throne the first time as RNG manipulation to manipulate away some unwanted event? You could have walked towards a wall, wallwalked, and walked back, and it'd have been faster in realtime (wallwalking is faster than sitting).
* The first attempted polymorph when on the throne enters an invalid polyform the first time round, presumably as a result of a typo. This synced with my script as no backspace was involved, but it's clearly ridiculously sloppy. (Invalid polyforms don't affect the RNG, so it's just completely a timewaste.)
* OK, I see why you slaughtered the throne room; it's so that you could waste time on the level without being interrupted. Not that that makes more sense than waiting on an already-cleared level and then luck-manipulating the throne. (Note that the longer you wait on the same level, the laggier it gets as more monsters spawn, so even better would be to split waiting time among a range of levels.)
* One of the spells you cast against the sergeants missed, to no benefit that I can see. You shouldn't be missing attacks in a TAS unless they're part of a larger sequence, if you're trying to anger a monster without hurting them, or if you're trying to hit some monsters and miss others that are in the line of fire.
* Magicbane strikes me as an odd choice of weapon for TASing. It has a lot of text, and many of its specials don't seem very helpful.
* "You can't go up here." You really should be loadstating away typos.
* I can't see a good argument for learning identify in a TAS. You should know what everything is already (e.g. quit-ID), and identifying the wand of cancellation does not seem useful at all. This is 3.4.3, so the name-to-write glitch is available and thus there likely isn't a benefit to formal IDs for scroll writing (you'd have to do it a lot with many different types of scroll to gain back the time you spent writing the ID spellbook).
* The PYEC gives MR, thus Magicbane's MR is redundant, meaning it mostly just has disadvantages (especially as you enchanted it up). You should really be using something like Greyswandir instead.
* Why enter the Mines at all in a TAS? Candles are wishable and it's a lot faster.
* Running around in circles on dlvl 12 confuses me a lot, given that you decided to gain speed earlier. These are basically two actions that cancel each other out and add a ton of realtime in the process. (There are other spells you can use to enhance escape.)
* You should be manipulating away Magicbane's probe effect. The time spent to manipulate it is minor compared to the time it wastes printing messages.
* You spend a surprising amount of time failing to open doors. This has rather more text than waiting.
* You're declaring attacks on peacefuls and cancelling them when you had a wall you could walk against instead. Again, this is only really explicable as a typo.
* What is the goal of this run? It started as gametime, became allegedly realtime, but the wish for the pony seems more like a playaround action than anything else (because you never use it).
* Home:1 is a pretty busy place to burn turns until T:2000, especially as there are monsters moving around within view. (You could at least have unequipped the Eyes of the Overworld. It looks like you've made no attempt at lag management at all.) This also reduces the entertainment of the run somewhat as it looks like you arrived on Home:1 early to be sure of getting there in time (whereas you'd imagine most TAS categories would arrive almost at the last moment).
* Dragging Rodney up with you is likely slow due to messages. (I can understand letting him spawn as it'll inevitably happen on Earth unless you let him spawn first and drag or paralyze him, but manipulating him away in Gehennom and letting him spawn on Earth is still probably faster than dragging him.)
* Why use the c!oGL route for the ascension run? I can understand why you'd want to avoid the complex amuletportation glitch used in the 2003 turn route, but the amulet branchport trick used in the 2006 turn route is easy enough to set up that it was tested in a live unassisted run shortly after discovery (i.e. a run that was already at the appropriate point modified its strategy to incorporate the trick). This is another reason not to go to Minetown (you'd want to do only 1 level of the Mines). This would also let you save on potion collecting earlier.
* c!oGLing into a vault with autopickup on is a bad idea. One wallwalk would probably land you somewhere with no text.
* I know getting a perfect portal on Fire is hard, but that one looks a little worse than average to me. You could almost certainly have done better. (I saw some signs of luck manipulation there, but it's hard to believe that it was cost-ineffective to do more.)
* If you're going to let liches hit you, why even have potions in inventory at this point? You could drop/box some or, preferably, make fewer. And if you're going to let the liches break the potions, you could at least skip the messages.
* You completed the game with unused wishes, despite wasting time to get wishes at earlier points in the game.
* Is there some way to stop the Angels using attacks with slow animations against you?
This doesn't look like a TAS. It looks more like a realtime speedrun than anything else (and in fact, it mostly looks single-segment rather than segmented).