Wow it's been almost 3 years since my last post in this thread, it seems like so long ago...
Anyway, I'm looking at implementing the known improvements in this run and seeing if I can find any of my own. If anyone is interested feel free to jump in.
Right now I'm just working on getting the known improvements all in one file. I saved 32 frames in 2-2, not sure exactly what Nitrodon's improvement was but it's probably that.
EDIT:
http://tasvideos.org/userfiles/info/47952051421383948
This file contains all the known improvements mentioned above. Now I'll start trying to find some more.
EDIT2: Found a pretty big improvement in 8-1. Nothing before that.
EDIT3:
http://tasvideos.org/userfiles/info/47959562881352562
Here is a file with the new 8-1 and also a bit of cleanup where I missed starting on the correct frame a couple times.