This game is annoying. Saved about 40 frames in the first 2 levels, then it was reduced to 2 frames in the middle of level 3, and then I saved a bit more and level 4 starts 15 frames sooner.
https://tasvideos.org/UserFiles/Info/637816761628744912
I couldn't find any invisible turtles in the first 3 levels.
Maybe object patterns can be manipulated to change, but I only got slight shifting by adding frame delays before the level starts.
This game requires a certain optimization technique. You can only move on a square grid. But platforms move slowly. So in places where you have to wait, you need to adjust your timing to be able to get closer to the very edge of the platform. Then when you arrive to a new platform, you're now closer to it, so you can jump on it sooner.
In addition to that, one should test various paths, because objects on the road can allow for a whole bunch of paths, and one can be faster than another.
MrTASer, would you like to try beating my times and learn techniques I used to beat yours?
PS: Figuring out object hitboxes can indeed be really hard if you don't know assembly language and debugging. I'd rather just manually test those things here.