This is the most optimal route I've made so far. This version took a lot of time to finish. This relies on current $1C (frame rule) position and how I should live with it. I call this (W1 + Item 1 and W2/W3 big refill timing abuse version). I forgot to mention that if I choose not to use one extra Item 1 at W1, my frame rule position is pretty bad and I'm going to lose a lot of time. Even picking a refill up from having higher amount of energy won't save you.
F = 0 (0 frames ahead) - Stylistic changes
H = 0 (0 frames ahead) - Stylistic changes
A = 10 (10 frames ahead) - Menu exploit, subpixel tweaks to avoid getting hit and earlier DelayRightScroll
C = 8 (18 frames ahead) - New boss fight
Q = 16 (34 frames ahead) - Crash boss glitch allows partially skip a first screen
M = 0 (34 framed ahead) - Stylistic changes
B = 39 (73 frames ahead) - Used Item 2 at the beginning, abuse to have 1 energy for spike zips and DelayDownScroll setup
W = 13 (86 frames ahead) - Switch immediately to H and perform DelayUpScroll (In the past this was proven impossible for many years and I went back to investigate it at random)
W1 = 24 (110 frames ahead) - $1C was nice, thanks to earlier stages
W2 = 0 (110 frames ahead) - Big refill "not doing a full charge" for $1C optimizations
W3 = 0 (110 frames ahead) - Big refill "not doing a full charge" (same $1C but extra Item 1 = no idea where to use it)
W4 = 0 (110 frames ahead) - Menu exploit didn't help
W5 = 0 (110 frames ahead) - Menu exploit didn't help
W6 = 1 frame saved (111 frames ahead) - Menu exploit galore
Total saving: 111 frames
Note: I still have an extra Item 1. It should be used starting from W3 stage. W3 is problematic cause it can save around 10 frames, I need a lot more to beat a 16 frame rule. Two Item 1s can do that but that saves 16 frames and using Item 1 at Alien fight saves 27 frames alone. At W4 there's no room to use it and at final Item 1 screen it only gives a ton of lag where I tried to gain some subpixels. It cannot be used before W3 because I'm otherwise getting extended charging duration. It's extremely important not to do full charges if you need to bypass some unnecessary waiting and where $1C is getting in the way. At W2 I charged from 12 to 14 and used 3x Item1s after that. At W3 I charged from 11 to 14 as well.