77 frames saved:
http://dehacked.2y.net/microstorage.php/info/1946469167/extra-boost.fcm
Normally, it isn't possible to jump over the eyeball guys, but if they are moving toward you, you can just BARELY make it over and boost forward. I think there were two other enemies in your TAS that you could do the same thing with.
EDIT: interesting thing I noticed. against the eyeball enemies, if you are lower than them (they are jumping/you are on stairs) and facing away from them and attack, the back of your weapon will hit them immediately. Not sure if that's useful, but I'm thinking it could be.
EDIT2: I guess it turned out to be slightly useful:
http://dehacked.2y.net/microstorage.php/info/198561684/enemy-kill.fcm
10 frames saved on the first enemy in room 014. Maybe it's possible to do it faster, I'm not sure how though.
I also believe that pressing right every other frame is the fastest way to climb stairs.
I guess I should have read this thread a little earlier.... heh...noticed you found what I have already. :D
There is one thing I'd like to add about this TAS. You'd assume that doing an invincbility glitch would definitely be the fastest method, but most likely (because of damage boosting), doing the invincibility glitch will probably be a bad idea... well I'm thinking it will be good for some parts and bad for others. It seems to me like you have an awful lot of health and damage boosting will save quite a lot of time in the long run.
Also, if you go left from the start of the game, will that area lead you to the end of the game or just items?