With Wart glitch, I improved my published run 182 frames.
For this glitch working, I can't use the 6th vegetable hit Wart at first time, must wait 1 frame. Then wait for 96 frames and hit Wart with the 7th vegetable.
#44954658645610208 - Super Mario All-Stars: Super Mario Bros. 2
Saved 64 frames in the first room and saved 3 frames in the vertical part. Unfortunately, I lost 53 frames on Birdo due to a different cycle. I have to wait for the last egg so I can't kill Birdo as fast as in my previous run. Overall, 14 frames saved in 5-2.
#29599425829190573 - Super Mario All-Stars: Super Mario Bros. 2 (5-1) new