In a series of vertical shoot 'em ups, Pop'n TwinBee: Rainbow Bell Adventures is a side-scrolling platformer with jetpack mechanics similar to Sparkster. Twinbee, Winbee, and Gwinbee must recover the seven Rainbow Bells and rescue Princess Melora from Dr. Warmon.

A translation of the in-game text in English is provided in the encodes as soft subtitles.