I watched about half of the tas so far, and a couple things have caught my attention.
1) I noticed that you are not making full use of the various items like the giant iron bar, or the concrete pole. When playing you can jump at them and hit them in mid air then on landing pick them up for use. See
https://youtu.be/9nhRkhXSDNM?si=mx2dGgHFDRtXr0Kh&t=49 . These weapons are very strong, but you do not make use of them at multiple points which loses a good amount of time. In fact not using it in the first level might be why you miss the Crusher at the end of the first stage for a quick stage 1 boss kill.
2) I have not seen any instances of the Button 1 + Button 2 special attacks. This would be faster to get a knockdown on enemies especially if you need to knock one in a hole.
3) One spot with some friendly fire was on frame 34000, where it looks like the motorcycle could have been killed faster.
4) Noticed how sometimes you should use other characters that are closer to enemies, but stick to using one character for the main attacking like at frame 34600 instead of a character that is closer to an enemy.
I have not seen this stated anywhere else so this would not count against the submission, but when I was testing the game I noticed that if you have two players that pincer an enemy you "catch" the enemy right after it takes a strong hit while the enemy is in mid air. Meaning instead of having an idle character at points the third one could get ready to jump in to do more damage.