The Lost Levels is a remake of the game released in Japan as Super Mario Bros 2. It introduces a number of new obstacles into the standard Super Mario Bros. formula, including poisonous mushrooms, wind, reverse warp zones, and of course many tricky jumps. It's difficult enough that Nintendo of America decided to completely re-theme a different game and release that as Super Mario Bros. 2 instead; The Lost Levels is the first time the Japanese sequel was released to the rest of the world.

This movie saves 00:02.83 seconds over the previous publication by using a few new tricks. All levels are played, including the secret world 9, and extremely difficult A through D worlds.