Nice job, evilchen.
In total you managed to save 432 frames compared to my 100% run when entering Fire Eye's village.
This was of course far too much to be a realistic improvement to my run, so I went ahead and compared both runs more carefully, trying to find a reason for this. And I did:
I actually did a horrible mistake at this point by not triggering this dialogue to start appearing much faster by simply pressing the action button 2 (not 1 time like I did in that case) times before it. This first few dialogues in the beginning were the only case where I didn't use turbo/autofire in the entire TAS to fast-forward them, because I at first planned to do that manually by pressing the action button on the first possible frame (but later realized that it would be too much unnecessary work to do so, so I started to use turbo a few seconds later after that). This one and only dialogue-delay in my entire run costed 283 (+/- 5) frames alone compared to your run. :-(
The real time saver you managed to get were ~1 sec in the first jungle room by skipping the Root pickup at the beginning and not killing another Wimpy Flower, and around 1,25 sec in the Raptor fight by manipulating their position somewhat better when hitting them.
Also, you obviously saved a few frames by skipping the name entrance screen. I wouldn't do that (because it will look stupid in the entire run whenever your name gets mentioned), but everybody has his own opinion. I don't expect it to save much more than a few seconds in the entire run anyway.
Your run looks great so far, and I'm looking for more progress. Good luck!