Super Mario Bros. Deluxe (1999) is the Game Boy Color version of the original Super Mario Bros. In addition to the standard game, it features some new unlockable modes, such as a challenge mode and "You vs. Boo", where Mario must race Boo through several levels which have been modified to make them more interesting in a race.
In this movie, got4n and TehSeven complete the You vs. Boo mode, beating Boo in all eight levels, and over half a second faster than the previous run.
Note: Although this run appears to be longer than the previous movie, that run's time was calculated with an incorrect frame rate. This run is indeed faster.
This run starts from a save file that has beaten the game and collected 100,000 points in challenge mode to unlock the "You vs. Boo" mode. Runs that start from SRAM are not normally allowed; see the Movie Rules for more on this.
You can watch the complete run played back on console