This is a 17 frames improvement over my previous run. 21 frames were saved in 4-2, and 1 frame for 8-4 2nd room, and 5 frames were lost in the last room due to bad Podoboo RNG.
In my previous attempt, Luigi hit the hidden coin block and then Mario used it as a stool to reach the vine, which I already knew was unnecessary because even without the coin block they were at a high enough level to hit or reach the vine. The reason I overlooked this is that if you scroll the screen too far to the right before the vine grows enough, Mario won't be able to grab it. My previous TAS was spending too much time waiting for the vine to grow, so I improved on it and managed to gain a 1 frame rule.
And only simple improvement and loss were made in 8-4. I would be glad if someone could make a perfect movement up to the last axe without losing any time.