Metroid completed as fast as possible without holding back, and without concern for game sanity.
Uses a game restart sequence (up+a on the second controller) to save time in backtracking.
Much of this 34-second improvement comes from taking an unexpected route through Kraid's lair: the doors are tricked into teleporting Samus to different rooms with the help of the door glitch. Another substantial improvement came from using a new strategy in the statue room.
See Metroid Tricks for more information about the door glitch as well as other tips and tricks.